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Additional info for Axonal Branching and Recovery of Coordinated Muscle Activity after Transection of the Facial Nerve in Adult Rats
5 mA). 09 ms; (n=5). However, both latency and threshold values were dependent on slight differences in electrode position, and were thus disregarded for statistical analysis. The CMAP consisted of a fast 3-phasic positive-negative-positive wave, followed by a slow and not so prominent negative wave (Fig. 12A). With the recording procedure used here, the faster negative wave reﬂected the overlapping depolarizations of all innervated muscle ﬁbers. 14 ms; n=5 rats) of the negative wave was due to the similar conductance velocities and excitability of the involved ﬁbers.
Reprinted from Tomov et al. , manuscript in preparation). , Englewood, CO, USA). The spatial model consisted of three reference points (Fig. 5): 1. A point in the medial sagittal line Fr-Occ (perpendicular to a line connecting both orbits) close to the end of the nose. We selected this reference point because the permanent snifﬁng movements of the nose tip would jeopardize the measurements. 2. A point corresponding to the medial angle of the left (s) orbit. 3. A point corresponding to the medial angle of the right (r) orbit.
Subgroup F2 consisted of 24 blind adult female Sprague-Dawley (SD) rats (substrain Royal College of Surgeons, RCS). The RCS rats lose their photoreceptor cells 2 weeks after birth due to a genetic defect of the retinal pigment epithelium (Sheedlo et al. 1991; D’Cruz et al. 2000;). Thus, the mystacial vibrissae are the only available means for these animals to receive and resolve spatial information (Brecht et al. 1997). 28 Materials and Methods: Experimental Sets These animals were used in three experimental sets.